S. aureus MurD Assay Kit Plus-100

Mfr.No.	MURD100KS
Description	MurD is a D-Glutamic acid-adding enzyme in the pathway for bacterial cell-wall peptidoglycan synthesis. It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurD catalyses the addition of D-glutamic acid to UDP-MurNAc-L-Ala, generating UDP-MurNAcdipeptide. The ligation reaction uses ATP hydrolysis as an energy source forming ADP and inorganic phosphate. The S. aureus MurD Assay Kit Plus-100 (Catalog No. MURD100KS) contains the reagents for 100 assays in a 384-well plate assay format including 400 µl of 10 x Buffer, 33 µl of 100 x UDP[1]MurNAc-L-Ala (UMA), 33 µl of 100 x D-Glu, 33 µl of 100 x ATP , 33 µl of 100 x S. aureus MurD (2000 nM) and 5 ml of Dye MPA3000 for phosphate detection.
Drug target assays	Antibacterial drug targets

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on the 384-well plate assay format. The reaction volume is 30 µl and the final assay volume is 75 µl. For 96-well plate assays, the reaction volume is 60 µl and the final assay volume is 150 µl. For detection using a cuvette, the reaction volume is 400 µl and the final assay volume is 1000 µl. 1.Reagent preparation: For each 10 assay reactions, (1) Prepare 297 µl of premix composed of 261 µl of H2O, 33 µl of 10 x Buffer and 3.3 µl of 100 x S. aureus MurD. (2) Prepare 33 µl of 10 x Enzyme substrate by mixing 3.3 µl of 100 x UMA, 3.3 µl of 100 x D-Glu, 3.3 µl of 100 x ATP, and 23.1 µl of water. 2.Reaction: Mix 27 µl of the premix with 3 µl of the 10 x Enzyme substrate in each well. Incubate the reaction mixture at 37°C for 60 min. 3. Detection: Add 45 µl of the Dye MPA3000 into the 30 µl of the reaction mixture. Incubate for 5 min. Measure the light absorbance at 650 nm.

Have you used this product?

SKU	Supplier No.	Size	Your price	Quantity
BHE16300044	MURD100KS	100 assays	$634.60		Add to Cart