rVSV pseudotyped Sudan Virus GP (Gulu Strain) or Recombinant Vesicular Stomatitis Virus pseudotyped Sudan Virus Gulu glycoprotein (rVSV-SUDV-Gulu-GP)) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSVpseudotyped virus containing the envelope glycoprotein of Sudan virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.