Loading ....
RNAi Service
RNA interference is a very important technology to study the function of specific genes in both in vitro and in vivo experiments and has been employed to develop RNAi therapies. Viral vectors, such as AAV/Adenovirus/Lentivirus are well-established systems to deliver RNAi through the expression of shRNA, with excellent safety, efficiency, and specificity.
Comparison of RNAi and CRISPR/Cas9
Both RNAi and CRISPR/Cas9 are methods for down-regulating gene expression. So, how to choose between these two methods?
RNA interference knocks down the target gene at the mRNA level, while Cas9 knocks down the gene at the genomic level. Cas9 can completely eliminate the expression of the target gene, and the function of the target protein is therefore completely lost.
| Comparison | RNAi | CRISPR/Cas9 gene knockout |
| Scope of interference | mRNA level | Genomic level |
| Target range | Only transcript | All genome sequences, such as exons, introns, promoters, enhancers, intergenic sequences, etc. |
| Elimination level of target protein | Incomplete elimination | Complete elimination |
| Function of target protein | Lost in part | Completely lost |
The services we provide include shRNA design, shRNA cloning, RNA interference efficiency screening, virus packaging (Lenti-Virus, Ad-Virus, AAV, and more) as well as the construction of stable cell lines.
shRNA Design and Vector Construction
● shRNA is designed based on the target gene, which is driven by pol III promoters (H1, U6).
● The inducible shRNA expression plasmid was constructed by combining with the Cre-LoxP system, which could achieve specific interference.
● The shRNA plasmid based on miR30 structure can achieve specific interference, which is driven by pol II promoters (CMV, EF1a, CAG, TH, GFAP, etc.).
Guaranteed shRNA Knockdown
A set of three expression plasmids are offered against every target gene with the guarantee that at least one of the three will knock down corresponding gene expression by 70% or more as determined by qRT-PCR. If not, the constructs will be replaced one time free of charge.
Markers and Reporters
A puromycin marker is used to select stable cells. The vectors contain mCherry or eGFP reporter genes to monitor transfection efficiency or transduction efficiency.
Delivery
● 3 individual shRNA constructs of 5 µg purified plasmid.
● shRNA screening report
● Lentivirus-shRNA or AAV- shRNA and a scrambled control virus.
We have extensive experience in RNAi and could offer optimum experimental conditions according to your requirements. If you have any needs, just email us at [email protected].
shRNA Design and Vector Construction
● shRNA is designed based on the target gene, which is driven by pol III promoters (H1, U6).
● The inducible shRNA expression plasmid was constructed by combining with the Cre-LoxP system, which could achieve specific interference.
● The shRNA plasmid based on miR30 structure can achieve specific interference, which is driven by pol II promoters (CMV, EF1a, CAG, TH, GFAP, etc.).
Guaranteed shRNA Knockdown
A set of three expression plasmids are offered against every target gene with the guarantee that at least one of the three will knock down corresponding gene expression by 70% or more as determined by qRT-PCR. If not, the constructs will be replaced one time free of charge.
Markers and Reporters
A puromycin marker is used to select stable cells. The vectors contain mCherry or eGFP reporter genes to monitor transfection efficiency or transduction efficiency.
Delivery
● 3 individual shRNA constructs of 5 µg purified plasmid.
● shRNA screening report
● Lentivirus-shRNA or AAV- shRNA and a scrambled control virus.
We have extensive experience in RNAi and could offer optimum experimental conditions according to your requirements. If you have any needs, just email us at [email protected].
ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.
© 2024 Biohippo. All rights reserved.