This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Host
Mouse
Immunogen
Recombinant full-length human MART-1 protein
Isotype
IgG2b Kappa
Positive Control
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Reactivity
Human, Horse
Recombinant
TRUE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
Q16655
Gene Id
2315
Clone No
rMLANA, 1905
Symbol
MLANA
Weight
20-22kDa (doublet)
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
FACS, IF, WB
Description
Optimal dilution for a specific application should be determined., FACS ; IF ; WB
Dilution
Flow Cytometry
0.5-1μg/million cells
Immunofluorescence
1-2μg/ml
Weste Blotting 0.5-1μg/ml)
Reviews of Anti-undefined Antibody rMLANA,1905 Unconjugated BHA10208245