Anti-undefined Antibody rGROEL,780 Unconjugated BHA10208226

Mfr.No.	3329-MSM7-P1BX
Alternative Names	60kDa chaperonin, 60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)
Categories	Primary Antibodies
Cellular Localization	Cytoplasm, Mitochondria
Chromosome Location	2q33.1
Clonality	Monoclonal
Description	Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone rGROEL/780, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
Host	Mouse
Immunogen	Recombinant full-length human HSP60 protein
Isotype	IgG1 Kappa
Positive Control	HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.
Reactivity	Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey, Chicken, Xenopus laevis, Drosophila
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P10809
Gene Id	3329
Clone No	rGROEL, 780
Symbol	HSPD1
Weight	60kDa

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Applications

FACS, IF, WB, IHC-F

Description

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

Flow Cytometry	0.5-1μg/million cells
Immunofluorescence	0.5-1μg/ml
Western Blotting	0.25-0.5μg/ml
Immunohistology (Formalin-fixed)	0.5-1μg/ml for 30 minutes at RT