Anti-undefined Antibody MYOD1,2075R Unconjugated BHA10208001

Mfr.No.	4654-RBM5-P0
Alternative Names	bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	11p15.1
Clonality	monoclonal
Description	Recognizes a phospho-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Host	Rabbit
Immunogen	Recombinant full-length human MyoD1 protein
Isotype	IgG
Positive Control	Fetal skeletal muscle or Rhabdomyosarcoma
Reactivity	Human
Recombinant	TRUE
Regulatory	RUO
Species	Homo Sapiens (Human)

Swissprot	P15172
Gene Id	4654
Clone No	MYOD1, 2075R
Symbol	MYOD1
Weight	45kDa

Buffer	200μg/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200μg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml
Storage	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Applications

IHC-F

Description

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined., IHC-F

Dilution

Immunohistology (Formalin-fixed)

0.5-1μg/ml for 30 minutes at RT