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Integrated AAV Offerings: From Cutting-Edge Products to Advanced Custom Services
Overcoming AAV Technology Challenges with Expertly Tailored Vector Design and Production
Premade AAV Vectors for Gene Delivery
Premade AAV vectors offer a convenient solution for researchers conducting gene delivery experiments, such as gene therapy, gene editing, and gene regulation. At Biohippo, these vectors are available in a variety of serotypes, promoters, and reporters/transgenes, allowing researchers to select the most suitable vector for their specific research needs. With over a thousand known AAV serotypes, researchers can efficiently determine vector specificity for different cell types. Additionally, the availability of multiple promoters with reporter genes enables easy monitoring and tracking of gene expression levels and timing.
To ensure the highest quality and purity, our partners employs various assays to evaluate their premade AAV vectors. These include qPCR titer assays to measure the concentration of AAV particles carrying the desired transgene, endotoxin testing to assess bacterial contamination, SDS-PAGE gel analysis to determine particle purity, and infectivity assays to evaluate the efficiency of cell transduction. The high purity and quality of these premade AAV vectors offer several advantages, including reduced optimization, less labor, and minimized expertise requirements, ensuring reliable and consistent results for both in vitro and in vivo applications.
We extensive catalog features over 2,000 premade AAV vectors, offering combinations of different genome types (ssAAV or scAAV), more than 22 serotypes, over 50 promoters (CAG, CMV, EF1a, hSyn, GFAP, NSE, CaMKII, etc.), fluorescent proteins (EGFP, mCherry, TdTomato), and reporters (Gluc, Cluc, Fluc, Rluc). These vectors are available for individual purchase or as part of a testing kit. Additionally, we offer custom AAV design and packaging services, capable of producing any known serotype or variant tailored to research needs.
Premade AAV Serotypes and Target Tissues/Cells
The following table provides a view of premade AAV serotypes that are suitable for targeting specific tissues and cell types, helping researchers choose the most appropriate vectors for their studies.
Target Tissue/Cell | Effective AAV Serotypes |
---|---|
Adrenal glands | AAV9, AAV10 |
Renal tissue | AAV9 |
Skeletal muscles | AAV1, AAV2, AAV4, AAV6, AAV8, AAV9, AAV10, AAV11 |
Cardiomyocytes | AAV1, AAV6, AAV8, AAV9 |
Endothelial vascular smooth muscle | AAV1, AAV2, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9 |
Inner ear cells | AAV1, AAV2, AAV6, AAV8 |
Retinal cells | AAV1, AAV2, AAV5, AAV8, AAV9, AAV10 |
Central Nervous System (CNS) | AAV1, AAV2, AAV5, AAV8, AAV9, AAV10 |
Airway Epithelium | AAV2, AAV5 |
Hepatocytes | AAV2, AAV8 |
Salivary Glands | AAV2, AAV9 |
Pancreatic cells | AAV1, AAV2, AAV8 |
Small intestine cells | AAV4, AAV5, AAV9 |
Colon cells | AAV5, AAV9 |
Lymph nodes | AAV9, AAV10 |
Testis cells | AAV4, AAV9 |
Newly added AAV Serotypes and Target Tissues/Cells
The following table provides a view of on new serotypes that have specific targeting capabilities, particularly for the central nervous system and brain-related applications.
New Serotype | Target Tissue/Description |
---|---|
AAV/11 | Efficient retrograde targeting of projection neurons. |
AAV/B10 | Central Nervous System (CNS), suitable for the central nervous system, can cross the blood-brain barrier. |
AAV/B22 | Central Nervous System (CNS), suitable for the central nervous system, can cross the blood-brain barrier. |
AAV/Mac | Efficiently transfects the central nervous system of non-human primates and can cross the blood-brain barrier (intravenous injection). |
AAV/BR1 | Targeting brain vascular endothelial cells. |
AAV/BI30 | Targeting brain vascular endothelial cells. |
All the AAV serotypes listed above, including the newly available options such as AAV/11, AAV/B10, AAV/B22, AAV/Mac, AAV/BR1, and AAV/BI30, can be selected when ordering our premade rAAVs. These serotypes are designed to target specific tissues and cells, providing you with versatile options for your gene delivery and research needs. Whether you're focusing on CNS applications, retrograde targeting, or vascular endothelial cells, we have a suitable AAV serotype ready to support your project.
Request and Production Guide
Submit your needs to us by clicking the AAV Service Forms in the side menu or email us at [email protected] and our scientific and production team will design your vector and provide you with a price quote. Or, for any customers who decide to make use of viruses as nucleotide sequence delivery tools, our scientists will work with you step by step from the design of a study to viral manufacturing to facilitate your research.
AAV Products categorized by function
Our pre-made AAVs are categorized by functions such as Biosensors, Chemogenetics, Controls, Optogenetics, and Recombinases, aiding researchers in selecting the appropriate AAV products for different research applications.
Chemogenetics AAV
Chemogenetics is an innovative technique that combines genetic engineering with the use of specific chemical compounds to control cell activity, particularly in neuroscience and cell signaling studies. Chemogenetics AAV (Adeno-Associated Virus) vectors are designed to deliver genes encoding engineered receptors, such as Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), into targeted cells or tissues. These engineered receptors are inert under normal physiological conditions but can be selectively activated or inhibited by synthetic ligands, allowing precise control over neuronal activity, signaling pathways, and cellular functions.
Chemogenetics AAV | ||||
---|---|---|---|---|
Receptor | Promoter | Tag | Activity | Serotype |
Activating | Synapsin | Fusion tags | Cre-dependent | AAV1 |
hM3D(Gq) DREADD | CaMKIIa | dTomato | Flp-dependent | AAV2 |
rM3D(Gs) DREADD | CD68 | EGFP | Constitutive (non-cre dependent) | AAV5 |
Inhibitory | Dlx | HA | AAV8 | |
hM4D(Gi) DREADD | E2 regulatory element | mCherry | AAV9 | |
KOR DREADD | GFAP | mCitrine | Retrograde AAV | |
PSAM4 GlyR | Non-fusion tags | PHPeB |
Biosensor AAV
Biosensor AAV (Adeno-Associated Virus) vectors are powerful tools used to study dynamic biological processes in real time by delivering genes encoding biosensors into specific cells or tissues. These biosensors are genetically encoded fluorescent or luminescent proteins that can report changes in cellular environments, such as calcium levels, pH, voltage, neurotransmitter release, and other molecular interactions. When delivered using AAV vectors, these biosensors provide long-term and targeted expression in living cells or organisms, allowing researchers to monitor physiological changes with high precision.
Biosensor AAV | ||||
---|---|---|---|---|
Calcium Sensors | Promoter | Tag | Activity | Serotype |
GCaMP5G | CAG,CAMKIIa,CMV,CRH | dTomato | Cre-dependent | AAV1 |
GCaMp6s | EF1α,GfaABC1D,GFAP,hSyn | Flag-HA | Flp-dependent | AAV5 |
GCaMp6f | mTH,nEF1α,OT,OXTR | Cre and Flp-dependent | AAV8 | |
GCaMp6m | PV | Cre, Flp, and VCre-dependent | AAV9 | |
jGCaMp7 | thy1 | Constitutive (non-cre dependent) | Retrograde AAV | |
CaMPARI | TRE3g | PHPeB | ||
jRGECO1, jRCaMP1 | TRPV1 |
Neurotransmitter sensor
Neurotransmitter sensor AAV (Adeno-Associated Virus) vectors are specialized tools designed to monitor neurotransmitter dynamics in real time within the nervous system. These AAV vectors deliver genes encoding genetically encoded neurotransmitter sensors (such as G-protein-coupled receptor-based sensors) to specific neurons or brain regions. These sensors can detect the release and uptake of neurotransmitters like glutamate, dopamine, serotonin, acetylcholine, and others, providing a powerful means to study synaptic transmission, neural circuit activity, and neurochemical signaling.
Neurotransmitter sensor | ||||
---|---|---|---|---|
Neurotransmitter sensors | GABA Sensor | ATP Sensors | Other Sensors | Promoter |
Dopamine Sensors | iGABASnFR | ATP1.0 | CCK2.0 | CAG |
dLight1.1 | Acetylcholine Sensors | ATP1.0_mut | AVP1.5 | EF1α |
DA1m | ACh2.0 | ATP1.1h | eCB1.0 | GfaABC1D |
DA1h | ACh4.3 | ATP1.1m | MT1.5 | hSyn |
DA1h mut | ACh4.3_mut | Serotonin Sensors | SST1.5 | |
DA4.4 | Norepinephrine (NE) Sensors | 5HT2.1(mutant) | VIP1.0 | |
Glutamate Sensor | NE1m | 5HT2.1 | ||
iGluSnFR(A184S) | NE1h | 5HT2B | ||
iGluSnFR(A184S) | NEmut | 5HT6 | ||
iGluSnFR(A184S) | NE3.1 | |||
Adenosine Sensors | ||||
Ado1.0 | ||||
Ado1.0mut |
Optogenetics AAV
Optogenetics AAV (Adeno-Associated Virus) vectors are revolutionary tools in neuroscience and cellular biology that enable precise control of cellular activity using light. Optogenetics involves genetically encoding light-sensitive proteins, such as channelrhodopsins, halorhodopsins, or opsins, into specific neurons or cells. When these proteins are expressed via AAV vectors, they allow researchers to activate or inhibit targeted cells with millisecond precision by simply shining light of specific wavelengths. This technique provides unprecedented control over neural circuits, cellular signaling pathways, and physiological processes.
Optogenetics AAV | ||||
---|---|---|---|---|
Opsin | Promoter | Fluorophore | Activity | Serotype |
Excitatory | TBG | GFP | Cre dependent | AAV1 |
Wild-type ChR2 | CAG | EGFP | Flp dependent | AAV2 |
ChR2/H134R | CaMKIIa | EYFP | Cre and Flp dependent | AAV5 |
soCoChR | CMV | mCherry | Constitutive | AAV8 |
ChR2/other mutants | CS-CRM4-aMHC | tdTomato | AAV9 | |
Chronos, Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) | D1 | YFP | AAVrg | |
Inhibitory | D2 | citrine | PHPeB | |
Arch and variants | EF1α | mOrange | ||
eNpHR and variants | GFAP | |||
Jaws | hSyn | |||
stGtACR | Ki67 | |||
iC++ | L7 | |||
OPN3 | mDLx | |||
PPO | nEF1α | |||
Bidirectional | PTH | |||
BiPOLES | RAM | |||
TRE3g | ||||
TRPV1 | ||||
VGAT1 |
Recombinase
Recombinase AAV (Adeno-Associated Virus) vectors are advanced tools used in genetic engineering to achieve targeted and precise DNA recombination in specific cells or tissues. These vectors deliver genes encoding recombinase enzymes, such as Cre, Flp, or Dre recombinases, which recognize specific DNA sequences (loxP, FRT, or rox sites, respectively) to catalyze the deletion, inversion, or translocation of genetic material. Recombinase systems are widely used to control gene expression, study gene function, and create conditional knockout or knock-in models.
Recombinase | ||||
---|---|---|---|---|
Recombinases | Promoter | Tag | ||
Cre | CMV | EF1α | Mash1 | 3XFlag |
CAG | E-SARE | mCD68 | dsRed | |
CaMKIIa | ETV1 | mDlX | EGFP | |
CAR | foxb1 | Mecp2 | EYFP | |
CB1 | fPV | mOXT | Flag | |
CBh | fSST | mTH | mCherry | |
cfos | GAD67 | Oxytocin | ||
ChAT | GFAP | PTH | ||
CRH | GLP | PV | ||
CS-CRM4-αMHC | Grm6 | RK | ||
CX3CR1 | GRP | TBG | ||
D1 | hCD68 | TH | ||
D1(SP) | HDC | Tmem119 | ||
D2 | hSyn | TPH2 | ||
D2(ENK) | Hypocretin | TRE3G | ||
DBH | KI-67 | TRPV1 | ||
Dlx5/6 | L7 | VGAT1 | ||
VGAT2 | ||||
CreERT2 | cfos | Flag | ||
E-SARE | ||||
FLP | CMV | hSyn | CAG | EGFP |
Learn About Available Viruses at Biohippo
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Neural Circuit Tracing
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