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Ready-to-load PEGylated HSPC Liposomes with Ammonium Sulfate

BHE16300457

This product includes 10 ml liposomes with 10 mg/ml lipid concentration. The mass ratio of lipids is HSPC: cholesterol: DSPE-PEG2000 = 3:1:1. The concentration of ammonium sulfate encapsulated within the liposomes for drug loading is 200 mM. The buffer is 10 mM histidine, pH 6.5, 9.2 % sucrose. Liposomes are stored in a 2°C to 8°C refrigerator. DO NOT freeze liposomes.

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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
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Shipping Temperature Cold but not frozen
Product Manual Datasheet
Protocol DRUG LOADING PROTOCOL
 
Drug molecules containing at least one amine group are potentially loaded into the liposomes. The drug loading capacity depends on the property of the drug molecules. The typical drug to lipids ratio is from 1:5 to 1:20.
 
1. Drug loading
Mix the drug solution and the liposome and incubate the mixture in a 48°C water bather for 3 hours. Save the loaded liposomes at 2 to 8°C.
 
2. Drug encapsulation measurement
The yield of drug encapsulation is measured by a spin-column method using the Liposome Drug Encapsulation Assay kit (Catalog number LDE10). A volume of 50 µl of sample was loaded on the spin column and eluted with 100 µl of the elution buffer. The encapsulated dug is eluted and the non-encapsulated drug stays on the column.
SKU Supplier No. Size Your price Quantity
BHE16300457 PHP100AS 10 ml $331.38
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