D-Luciferin, Potassium Salt

Mfr.No.

40902ES02, 40902ES03, 40902ES01

Description

D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging Technology. The mechanism of action is that luciferin (substrate) can be oxidized to emit light in the presence of ATP and luciferase. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice.

D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are three Product form: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in solubility: water solubility of the former and solubility of the buffer system. They are weakly soluble except in weak bases such as low concentrations of NaOH and KOH solutions. Soluble in methanol and DMSO; The latter can be easily dissolved in water or buffer, easy to use.

Applications

In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.
The localization and distribution of foreign cells in animals were detected.
The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo

Alternative Names

(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt

Appearance

Light yellow powder

Features

No radiation, almost harmless to living organisms.
Bioluminescence, no excitation light source.
So sensitive, you can detect it in a few hundred cells.
Good penetration, 3-4cm tissue depth can still be detected.
High signal-to-noise ratio, strong fluorescence signal and good anti-interference.

Kit Components

Components No.	Name	40902ES01	40902ES02	40902ES03	40902ES08
40902	D-Luciferin, Potassium Salt	100mg	500 mg	1g	5g

Molecular Formula

C11H7N2O3S2K

Shipping

The product is shipped with dry ice.

Cas Number

115144-35-9

Molecular Weight

318.42 g/mol

Solubility

Soluble in water(60 mg/mL)

Storage	Stored at -15℃ ~ -25℃ for 1 year.

Product Manual	40902_MSDS_HB220415_EN_1652931562565.PDF 40902_MSDS_HB220422_EN_1652931562524.PDF 40902_MSDS_HB220422_EN_1652931562832.PDF Manual

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Combination SKU	Supplier No.	Size	Your price
BHB20800005-1	40902ES02	500 mg	$365	Add to Cart
BHB20800005-2	40902ES03	1 g	$445	Add to Cart
BHB20800005-3	40902ES01	100 mg	$195	Add to Cart

D-Luciferin, Potassium Salt

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