Matrices listed below were spiked with certain level of NOS3/eNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS3/eNOS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-100
97
EDTA plasma(n=5)
86-104
95
heparin plasma(n=5)
95-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS3/eNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NOS3/eNOS ELISA Kit allows for the in vitro quantitative determination of NOS3/eNOS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) ELISA Kit