Rat Factor-related Apoptosis,FAS ELISA Kit

Rat Factor-related Apoptosis,FAS ELISA Kit

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Mfr.No.

CSB-E07324r

Alternative Names

Fas (TNF receptor superfamily, member 6), ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, APO-1 cell surface antigen, CD95 antigen, Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily, member 6

Reactivity

Rat

Detection Range

0.78 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	101
	Range %	99-105
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.531	2.481	2.506	2.364
100	2.032	1.961	1.997	1.855
50	1.448	1.401	1.425	1.283
25	0.876	0.823	0.850	0.708
12.5	0.597	0.623	0.610	0.468
6.25	0.391	0.402	0.397	0.255
3.12	0.233	0.217	0.225	0.083
0	0.144	0.139	0.142	?

Species

Rattus norvegicus

Sensitivity

0.195 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q63199
Antigen	Fas (TNF receptor superfamily, member 6)

Applications

ELISA

Research Area

FAS

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507950	CSB-E07324r	96T	$695	Add to Cart
BHE10507950	CSB-E07324r	5x96T	$3336	Add to Cart
BHE10507950	CSB-E07324r	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.