Rat Factor-related Apoptosis,FAS ELISA Kit

Rat Factor-related Apoptosis,FAS ELISA Kit

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Mfr.No.

CSB-E07324r

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Fas (TNF receptor superfamily, member 6), ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, APO-1 cell surface antigen, CD95 antigen, Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily, member 6

Recovery

The recovery of human MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	101
	Range %	99-105
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.531	2.481	2.506	2.364
100	2.032	1.961	1.997	1.855
50	1.448	1.401	1.425	1.283
25	0.876	0.823	0.850	0.708
12.5	0.597	0.623	0.610	0.468
6.25	0.391	0.402	0.397	0.255
3.12	0.233	0.217	0.225	0.083
0	0.144	0.139	0.142	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q63199

Applications	ELISA
Research Area	FAS

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10507950	CSB-E07324r	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.