Rat Caspase-9,Casp-9 ELISA Kit

Rat Caspase-9,Casp-9 ELISA Kit

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Mfr.No.

CSB-E08863r

Alternative Names

Caspase-9,Casp-9

Reactivity

Rat

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	92	89-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-99
1:2	Average %	101
	Range %	93-105
1:4	Average %	94
	Range %	86-98
1:8	Average %	98
	Range %	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.093	1.952	2.023	1.940
1000	1.344	1.321	1.333	1.250
500	0.781	0.777	0.779	0.696
250	0.471	0.455	0.463	0.380
125	0.283	0.278	0.281	0.198
62.5	0.212	0.207	0.210	0.127
31.25	0.149	0.145	0.147	0.064
0	0.085	0.081	0.083	?

Species

Rattus norvegicus

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9JHK1
Antigen	Caspase-9,Casp-9

Applications

ELISA

Research Area

Casp-9

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Bacoside a Attenuates Nephrotoxicity and Acute Kidney Injury in Male Albino Rats Induced by Cisplatin Gong Xiao, et al,International Journal of Pharmacology,January 15

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.