Rat Caspase-9,Casp-9 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08863r

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Caspase-9,Casp-9

Recovery

The recovery of human UCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	92	89-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human UCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-99
1:2	Average %	101
	Range %	93-105
1:4	Average %	94
	Range %	86-98
1:8	Average %	98
	Range %	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9JHK1

Applications	ELISA
Research Area	Casp-9

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Bacoside a Attenuates Nephrotoxicity and Acute Kidney Injury in Male Albino Rats Induced by Cisplatin Gong Xiao, et al,International Journal of Pharmacology,January 15

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