Rat Apoptosis regulator BAX(BAX) ELISA kit

Mfr.No.

CSB-EL002573RA

Alternative Names

Apoptosis regulator BAX(BAX), BCL2L4, apoptosis regulator BAX

Reactivity

Rat

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse GLRX5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	106	102-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLRX5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-96
1:2	Average %	96
	Range %	92-100
1:4	Average %	84
	Range %	80-88
1:8	Average %	93
	Range %	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.639	2.524	2.582	2.431
1000	2.172	2.068	2.120	1.969
500	1.593	1.523	1.558	1.407
250	0.925	0.892	0.909	0.758
125	0.587	0.571	0.579	0.428
62.5	0.368	0.346	0.357	0.206
31.25	0.256	0.247	0.252	0.101
0	0.149	0.153	0.151	?

Species

Rattus norvegicus

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q63690
Antigen	Apoptosis regulator BAX(BAX)

Applications

ELISA

Research Area

BAX

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of ukrain on ischemia/reperfusion-induced kidney injury in rats Bekir Aras. et al,Journal of Surgical Research,2015 online Effect of Ukrain on Ischemia/Reperfusion-Induced Kidney Injury in Rats Aras B.et al,J Surg Res.,May 15 Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats Othman A.et al,Biomed Pharmacother.,/ Olmesartan ameliorates chemically-induced ulcerative colitis in rats via modulating NFκB and Nrf-2/HO-1 signaling crosstalk Saber S, et al,Toxicology and Applied Pharmacology,December 27

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SKU	Supplier No.	Size	Your price
BHE10507632	CSB-EL002573RA	96T	$695	Add to Cart
BHE10507632	CSB-EL002573RA	5x96T	$3336	Add to Cart
BHE10507632	CSB-EL002573RA	10x96T	$6602.50	Add to Cart

Rat Apoptosis regulator BAX(BAX) ELISA kit

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