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Home / ELISA Kit

Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit

SKU : BHE13700218

Activated CDC42 kinase 1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain.
The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

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Mfr.No.	KTE100064
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK2.
Alternative Names	TNK2; ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase
Detection Method	Colorimetric
Kit Components	Rat Activated CDC42 kinase 1 microplate Rat Activated CDC42 kinase 1 standard Rat Activated CDC42 kinase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNK2. No significant cross-reactivity or interference between Rat TNK2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Activated CDC42 kinase 1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
Notes	This Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK2 in samples. An antibody specific for TNK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	303882

Applications	ELISA
Applications Notes	This Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK2 in samples. An antibody specific for TNK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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