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Home / ELISA Kit

Rat 14-3-3 protein theta (YWHAQ) ELISA Kit

SKU : BHE13700150

YWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.The deduced 245-amino acid protein has a calculated molecular mass of 28 kD and shares 72% amino acid identity with bovine brain 14-3-3-eta. Northern blot analysis of Jurkat and HeLa cells showed strong bands at 1.9 and 2.1 kb, and a weaker band at 1.7 kb.

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Mfr.No.	KTE100001
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat 14-3-3 protein theta (YWHAQ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAQ.
Alternative Names	YWHAQ; 14-3-3; 1C5; HS1; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; protein tau; tyrosine 3/tryptophan 5 -monooxygenase activation protein; theta polypeptide
Detection Method	Colorimetric
Kit Components	Rat 14-3-3 protein theta microplate Rat 14-3-3 protein theta standard Rat 14-3-3 protein theta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat 14-3-3 protein theta (YWHAQ) ELISA Kit has high sensitivity and excellent specificity for detection of Rat YWHAQ. No significant cross-reactivity or interference between Rat YWHAQ and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	YWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.The deduced 245-amino acid protein has a calculated molecular mass of 28 kD and shares 72% amino acid identity with bovine brain 14-3-3-eta. Northern blot analysis of Jurkat and HeLa cells showed strong bands at 1.9 and 2.1 kb, and a weaker band at 1.7 kb.
Notes	This Rat 14-3-3 protein theta (YWHAQ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAQ in samples. An antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	100008930

Applications	ELISA
Applications Notes	This Rat 14-3-3 protein theta (YWHAQ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAQ in samples. An antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700150	KTE100001	48 T	$339		Add to Cart
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