Human/Mouse/Rat/Rabbit/Dog/Pig/Bovine/Horse Ubiquitin (Ub) (Competitive EIA) ELISA Kit

Mfr.No.

CEA164Mi

Description

This assay has high sensitivity for detection of Ubiquitin (Ub).
100% cross-reactivity of Ubiquitin (Ub) was observed among Human, Mouse, Rat, Rabbit, Canine, Porcine, Bovine, Equine.

Alternative Names

Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B

Reactivity

Human, Mouse, Rat, Rabbit, Dog, Pig, Bovine, Horse

Detection Method

Colormetric

Detection Range

4.94-400ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids

Assay Type

Competitive Inhibition

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Ubiquitin (Ub) and the recovery rates were calculated by comparing the measured value to the expected amount of Ubiquitin (Ub) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	78-104	93
EDTA plasma(n=5)	83-96	92
heparin plasma(n=5)	92-101	96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ubiquitin (Ub) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	88-105%	90-99%	94-103%	94-101%
EDTA plasma(n=5)	87-95%	79-92%	95-103%	89-101%
heparin plasma(n=5)	81-101%	90-98%	86-95%	88-101%

Features

Detects small molecule

Species

Human///Mouse///Rat///Rabbit///Dog///Pig///Bovine///Horse

Sensitivity

1.92ng/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ubiquitin (Ub) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ubiquitin (Ub) and unlabeled Ubiquitin (Ub) (Standards or samples) with the pre-coated antibody specific to Ubiquitin (Ub). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ubiquitin (Ub) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ubiquitin (Ub) in the sample.

Gene ID	7314
UniProt ID	P0CG47
Antigen	Ub
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Applications	ELISA
Research Area	Reproductive science
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin (Ub) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin (Ub) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10109097	CEA164Mi	96T*1	$648	Add to Cart
BHE10109097	CEA164Mi	96T*5	$2916	Add to Cart
BHE10109097	CEA164Mi	96T*10	$5508	Add to Cart

ELISA Kit for Ubiquitin (Ub) (Human/Mouse/Rat/Rabbit/Dog/Pig/Bovine/Horse)

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