Human/Mouse/Rat/Rabbit/Dog/Bovine Glucagon Like Peptide 1 (GLP1) (Competitive EIA) ELISA Kit

Mfr.No.

CEA804Mi

Sensitivity

4.26pg/mL

Detection Range

12.35-1,000pg/mL

Reactivity

Human, Mouse, Rat, Rabbit, Dog, Bovine

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity for detection of Glucagon Like Peptide 1 (GLP1).
100% cross-reactivity of Glucagon Like Peptide 1 (GLP1) was observed among Human, Mouse, Rat, Rabbit, Canine, Bovine.

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Reagent Diluent	1×300µL	Stop Solution	1×6mL
TMB Substrate	1×9mL	Instruction manual	1
Wash Buffer (30 × concentrate)	1×20mL

Recovery

Matrices listed below were spiked with certain level of recombinant Glucagon Like Peptide 1 (GLP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Glucagon Like Peptide 1 (GLP1) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	89-102	98
EDTA plasma(n=5)	91-105	101
heparin plasma(n=5)	98-105	101

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucagon Like Peptide 1 (GLP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	91-101%	80-105%	78-98%	78-102%
EDTA plasma(n=5)	87-103%	81-101%	97-105%	83-98%
heparin plasma(n=5)	78-94%	99-105%	95-102%	89-103%

Features

Detects small molecule

Species

Human///Mouse///Rat///Rabbit///Dog///Bovine

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucagon Like Peptide 1 (GLP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucagon Like Peptide 1 (GLP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glucagon Like Peptide 1 (GLP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glucagon Like Peptide 1 (GLP1) and unlabeled Glucagon Like Peptide 1 (GLP1) (Standards or samples) with the pre-coated antibody specific to Glucagon Like Peptide 1 (GLP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glucagon Like Peptide 1 (GLP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glucagon Like Peptide 1 (GLP1) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Protocol

Datasheet

UniProt ID	P01275

Applications	ELISA
Research Area	Endocrinology

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Specificity and sensitivity of commercially available assays for glucagon‐like peptide‐1 (GLP‐1): implications for GLP‐1 measurements in clinical studies Expression of cholecystokinin2-receptor in rat and human L cells and the stimulation of glucagon-like peptide-1 secretion by gastrin treatment Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model Anti-hiperglycemic Effect of Urena lobata Leaf Extract by Inhibition of Dipeptidyl Peptidase IV (DPP-IV) on Diabetic Rats Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model miR-200a reCavia (Guinea pig )lates Rheb-mediated amelioration of insulin resistance after duodenal-jejunal bypass Incretin effect of Urena lobata leaves extract on structure and function of rats islet β-cells Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats

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SKU	Supplier No.	Size	Your price
BHE10102775	CEA804Mi	96T*1	$648	Add to Cart
BHE10102775	CEA804Mi	96T*5	$2916	Add to Cart
BHE10102775	CEA804Mi	96T*10	$5508	Add to Cart

ELISA Kit for Glucagon Like Peptide 1 (GLP1) (Human/Mouse/Rat/Rabbit/Dog/Bovine)

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