rAAV-EF1a-DIO-hM4D(Gi)-mCherry-WPREs

Mfr.No.	PT-0043/BC-0155
Description	Cre dependent expression of the hM4D(Gi) gene for inhibitory DREADD manipulation from the ubiquitous Ef1alpha promoter, hM4D(Gi) is fused with mCherry at the C-terminus
Function	Chemogenetics
Promoter	EF1a
Insert Gene	hM4D(Gi)
Fluorophore/Tag	mCherry
Activity	Cre-dependent
Serotype	Available serotypes: AAV2/1; AAV2/5; AAV2/8; AAV2/9; AAV2/DJ; AAV2/Retro; AAV2/PHP.eB; AAV2/2; AAV2/6; AAV2/rh10; AAV2/PHP.S; AAV2/anc80L65; AAV2/B10; AAV2/olig001; AAV2/6.2FF; AAV2/BI30; AAV2/7m8; AAV2/BR1; AAV2/6m; AAV2/MG1.2; AAV1/2; AAV2/MaCPNS1; AAV2/MaCPNS2; AAV2/DJ8; AAV2/PHP.B
Titer	>=2.00E+12 vg/mL; For higher titer (>=1.00E+13 vg/mL), please inquire.
Storage	Should be stored at -80°C

Reference Paper	A NAc Tac1 Neural Circuit Mediates Aversion Z He, K Xi, K Liu, Y Yin, H Yu, L Liu, X He… - Available at SSRN … - papers.ss.com … LSL-tdTomato (1.50 × 109 particles ml-1) were purchased from BrainVTA (China). AAV- 261 EF1α-DIO-mCherry (AAV2/9, 1.47 × 1013 particles ml-1) was purchased from GeneChem 262 (China). 263 For functional analysis, AAV-EF1α-DIO-hM4D(Gi)-mCherry (AAV2/9, 1.044 … A cholinergic medial septum input to medial habenula mediates generalization formation and extinction of visual aversion RH Mu, XM Han, H Wang, SS Tang, Y Long, DH Yuan… - 2021 - researchsquare.com … image. To explore whether chemogenetic and optogenetic inhibitions of MS cholinergic neurons 155 disrupt formation of aversion generalization, cre-dependent AAV-Ef1α-DIO-hM4D(Gi)-mCherry 156 (Supplementary Fig. 4a) or AAV-Ef1α-DIO-NpHR-mCherry (Fig … Regulation of Cued Fear Expression via Corticotropin-Releasing-Factor Neurons in the Ventral Anteromedial Thalamic Nucleus Y Lv, P Chen, QH Shan, XY Qin, XH Qi, JN Zhou - Neuroscience Bulletin, 2021 - Springer … For mice that received AAV-EF1α-DIO-hM4D(Gi)-mCherry (8.57 × 10 12 genome copies per mL, OBiO), AAV-EF1α-DIO-hM3D(Gq)-mCherry (5.40 × 10 12 genome copies per mL, BrainVTA), AAV-EF1α-DIO-mCherry (5.05 × 10 12 genome copies per mL, BrainVTA), or … A Neural Circuit from Paraventricular Thalamus to Bed Nucleus of the Stria Terminalis for the Regulation of States of Consciousness During Sevoflurane Anesthesia JY Li, SJ Gao, R Li, W Wang, J Sun, LQ Zhang… - Available at SSRN … - papers.ss.com … To inhibit PVT-BNST neural circuit, AAVretro-hSyn-Cre was bilaterally injected into the BNST, and then AAV-EF1α-DIO-hM4D(Gi) … (200 nl, BrainVTA Co., Ltd., China) or AAV-CaMKIIa-mCherry-WPREs (control) was delivered into …

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The production lead time for packaging our pre-made plasmids into AAV is typically 3-4 weeks. Please check the 'DOCUMENTATION' to read the 'Sales Terms and Conditions for adeno-associated viruses (AAVs)' before placing the order.

Combination SKU	Supplier No.	Serotype	Size	Your price
BHV12400072-1	PT-0043/BC-0155	AAV2/1	100ul	$531	Add to Cart
BHV12400072-2	PT-0043/BC-0155	AAV2/2	100ul	$531	Add to Cart
BHV12400072-3	PT-0043/BC-0155	AAV2/5	100ul	$531	Add to Cart
BHV12400072-4	PT-0043/BC-0155	AAV2/6	100ul	$531	Add to Cart
BHV12400072-5	PT-0043/BC-0155	AAV2/6m	100ul	$531	Add to Cart
BHV12400072-6	PT-0043/BC-0155	AAV2/6.2FF	100ul	$531	Add to Cart
BHV12400072-7	PT-0043/BC-0155	AAV2/7m8	100ul	$531	Add to Cart
BHV12400072-8	PT-0043/BC-0155	AAV2/8	100ul	$531	Add to Cart
BHV12400072-9	PT-0043/BC-0155	AAV2/9	100ul	$531	Add to Cart
BHV12400072-10	PT-0043/BC-0155	AAV2/DJ	100ul	$531	Add to Cart
BHV12400072-11	PT-0043/BC-0155	AAV2/DJ8	100ul	$531	Add to Cart
BHV12400072-12	PT-0043/BC-0155	AAV2/Retro	100ul	$531	Add to Cart
BHV12400072-13	PT-0043/BC-0155	AAV2/PHP.eB	100ul	$531	Add to Cart
BHV12400072-14	PT-0043/BC-0155	AAV2/PHP.B	100ul	$531	Add to Cart
BHV12400072-15	PT-0043/BC-0155	AAV2/rh10	100ul	$531	Add to Cart
BHV12400072-16	PT-0043/BC-0155	AAV2/PHP.S	100ul	$531	Add to Cart
BHV12400072-17	PT-0043/BC-0155	AAV2/anc80	100ul	$531	Add to Cart
BHV12400072-18	PT-0043/BC-0155	AAV2/B10	100ul	$531	Add to Cart
BHV12400072-19	PT-0043/BC-0155	AAV2/olig001	100ul	$531	Add to Cart
BHV12400072-20	PT-0043/BC-0155	AAV2/BI30	100ul	$531	Add to Cart
BHV12400072-21	PT-0043/BC-0155	AAV2/BR1	100ul	$531	Add to Cart
BHV12400072-22	PT-0043/BC-0155	AAV2/MG1.2	100ul	$531	Add to Cart
BHV12400072-23	PT-0043/BC-0155	AAV1/2	100ul	$531	Add to Cart
BHV12400072-24	PT-0043/BC-0155	AAV2/MaCPNS1	100ul	$531	Add to Cart
BHV12400072-25	PT-0043/BC-0155	AAV2/MaCPNS2	100ul	$531	Add to Cart

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rAAV-EF1a-DIO-hM4D(Gi)-mCherry-WPREs

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A NAc Tac1 Neural Circuit Mediates Aversion

A cholinergic medial septum input to medial habenula mediates generalization formation and extinction of visual aversion

Regulation of Cued Fear Expression via Corticotropin-Releasing-Factor Neurons in the Ventral Anteromedial Thalamic Nucleus

A Neural Circuit from Paraventricular Thalamus to Bed Nucleus of the Stria Terminalis for the Regulation of States of Consciousness During Sevoflurane Anesthesia

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