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Home / ELISA Kit / QuickTest ELISA

Rat Pdk1 (Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial) QuickTest ELISA Kit

SKU : BHE10811176

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Wuhan Fine Biotech Co., Ltd

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Mfr.No.	QT-ER0425
Description	3-phosphoinositide-dependent protein kinase 1\|hPDK1\|PDPK1\|PDK1
Reactivity	Rat
Detection Method	Double Antibody
Detection Range	0.156-10ng/ml
Sample Type	Cell lysates, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants
Assay Type	Sandwich
Assay Duration	120 minutes
Kit Components	Coated ELISA plate, standard, ready to use capture/detection antibody, TMB, sample dilution buffer, wash buffer, stop solution
Recovery	80%~120%
Sensitivity	0.094ng/ml
Specificity	specifically binds with Pdk1, no obvious cross reaction with other analogues.
Assay Procedure Summary	Step 1: Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C. Washing: Wash the plate twice without immersion. Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C. Washing: Wash the plate five times without immersion. Step 3: Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.) Step 4: Add 50ul stop solution. Read at 450nm immediately and calculate.
Stability	>95% at 4°C for 6 months

UniProt ID	Q63065
Antigen	Pdk1
Usage Notes	Microplate reader,incubator,automated plate washer or multi-channel pipette, precision single and multi-channel pipette,sterile tubes and eppendorf tubes, absorbent paper and loading slot, deionized or distilled water

Precision	Intra assay<8%,inter assay<10%

Storage	2-8 °C(for sealed)

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SKU	Supplier No.	Size	Your price	Quantity
BHE10811176	QT-ER0425	48T	$315		Add to Cart
BHE10811176	QT-ER0425	96T	$450		Add to Cart

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