Rat BDNF PicoKine® Quick ELISA Kit

Mfr.No.

FEK0308

Sensitivity

<15 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.014	0.039	0.076	0.120	0.255	0.485	1.020	1.955

Description

Rat BDNF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Bdnf in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bdnf; Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Immunogen

Expression system for standard: NS0; Immunogen sequence: H129-R247

Kit Components

Description Quantity Volume
Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
HRP-Linked Antibody 1 6ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Rat Bdnf

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	95	302	947	88	289	1024
Standard deviation	4.94	16.91	72.91	4.75	21.67	86.01
CV (%)	5.2%	5.6%	7.7%	5.4%	7.5%	8.4%

Background

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	95	98	94	105	98	6.17	6.3%
Sample 2	302	286	265	296	287	12.34	4.3%
Sample 3	945	983	914	962	951	46.59	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	24225
Gene Name	CST3
UniProt ID	P23363
Protein Name	Brain-derived neurotrophic factor

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Caspases,Cell Biology,Cell Type Markers,Neural Stem Cells,Stem Cells,Tags & Cell Markers,Tumor Associated

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000219	FEK0308	96 wells/kit	$499		Add to Cart

Rat BDNF PicoKine® Quick ELISA Kit

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