Mouse IL-2/Interleukin-2 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0398

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.033	0.102	0.200	0.399	0.634	1.357	1.726	2.042

Description

Mouse IL-2/Interleukin-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Il2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-2;IL-2;T-cell growth factor;TCGF;Il2;Il-2;

Molecular Weight

19400 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-Q169

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse IL-2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	37	156	467	40	166	452
Standard deviation	2.1	8.58	37.7%5	2.6	10.62	37.06
CV (%)	5.7%	5.5%	7.7%	6.5%	6.4%	8.2%

Protein Function

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

Background

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	37	40	36	39	38	1.58	4.1%
Sample 2	156	162	166	155	159	4.49	2.8%
Sample 3	467	448	470	463	462	8.45	1.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16183
Gene Name	Il2
UniProt ID	P04351
Protein Name	Interleukin-2

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000158	FEK0398	96 wells/kit	$499		Add to Cart

Mouse IL-2/Interleukin-2 PicoKine® Quick ELISA Kit

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