Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit

Mfr.No.

FEK0577

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.088	0.133	0.199	0.307	0.484	0.829	1.254	1.911

Description

Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Il6st in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-6 receptor subunit beta;IL-6 receptor subunit beta;IL-6R subunit beta;IL-6R-beta;IL-6RB;Interleukin-6 signal transducer;Membrane glycoprotein 130;gp130;Oncostatin-M receptor subunit alpha;CD130;Il6st;

Molecular Weight

102451 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-E617

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Gp130

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	159	1404	4588	158	1473	4382
Standard deviation	11.6	71.6	339.51	14.06	81.01	333.03
CV (%)	7.3%	7.6%%	7.4%	8.9%	5.5%	7.6%

Protein Function

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.

Background

GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	159	185	185	168	174	11.21	6.4%
Sample 2	1404	1352	1232	1273	1315	66.97	5%
Sample 3	4588	4716	4423	4674	4600	112.25	2.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16195
Gene Name	Il6st
UniProt ID	Q00560
Protein Name	Interleukin-6 receptor subunit beta
Tissue Specificity	Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.
Sequence	Belongs to the type I cytokine receptor family. Type 2 subfamily.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000201	FEK0577	96 wells/kit	$499		Add to Cart

Mouse Gp130/IL6ST PicoKine® Quick ELISA Kit

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