Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0977

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.035	0.071	0.139	0.260	0.384	0.666	1.120	1.877

Description

Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Crp in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-Reactive Protein/CRP; C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Immunogen

Expression system for standard: NS0; Immunogen sequence: H20-S225

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Mouse Crp

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	254	1752	6770	265	1851	6930
Standard deviation	11.93	94.6	453.59	12.98	105.5	512.82
CV (%)	4.7%	5.4%	6.7%	4.9%	5.7%	7.4%

Background

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	254	243	280	249	256	13.82	5.4%
Sample 2	1752	1563	1416	1662	1598	97.47	6.1%
Sample 3	6770	5545	5924	6118	6089	395.78	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	12944
Gene Name	CST3
UniProt ID	P14847
Protein Name	C-reactive protein

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Caspases,Cell Biology,Cell Type Markers,Neural Stem Cells,Stem Cells,Tags & Cell Markers,Tumor Associated

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000226	FEK0977	96 wells/kit	$499		Add to Cart

Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit

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