Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit

Mfr.No.

FEK1400

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Mouse

Sample Volume

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.002	0.313	0.609	1.052	1.626	2.217	2.290	2.478

Description

Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Complement Factor H/CFH in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S875-V1252

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Mouse Cfh

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1344	6741	29641	1352	6941	26413
Standard deviation	72.58	242.68	1304.2	51.38	423.4	1558.37
CV(%)	5.4%	3.6%	4.4%	3.8%	6.1%	5.9%

Background

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alteativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alteative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1344	1258	1323	1429	1339	84.33	6.3%
Sample 2	6741	6541	5974	6641	6474	407.88	6.3%
Sample 3	29641	26474	24165	26852	26783	1553.41	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	12628
Gene Name	Cfh
UniProt ID	P06909

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000305	FEK1400	96 wells/kit	$499		Add to Cart

Mouse Complement Factor H/CFH PicoKine® Quick ELISA Kit

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Reproducibility

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