Mouse BMP-2 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0313

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.002	0.016	0.027	0.056	0.179	0.415	1.118	2.016

Description

Mouse BMP-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Bmp2 in bone tissue, cell culture supeatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2;

Molecular Weight

44525 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse BMP-2

Subcellular Localization

Secreted.

Sample Type

Bone tissue homogenate, Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	62	249	770	61	243	710
Standard deviation	2.54	10.95	58.52	2.62	12.63	55.38
CV (%)	4.1%	4.4%	7.6%	4.3%	5.2%	7.8%

Protein Function

Induces cartilage and bone formation.

Background

Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	62	70	61	71	66	4.52	6.8%
Sample 2	249	252	246	234	245	6.83	2.7%
Sample 3	770	853	881	850	838	41.35	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12156
Gene Name	Bmp2
UniProt ID	P21274
Protein Name	Bone morphogenetic protein 2

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000181	FEK0313	96 wells/kit	$499		Add to Cart

Mouse BMP-2 PicoKine® Quick ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse BMP-2 PicoKine® Quick ELISA Kit

Rate it now..!!