Human MMP-8 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0464

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.017	0.056	0.119	0.257	0.464	0.918	1.576	1.937

Description

Human MMP-8 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human MMP8 in cell culture supeatants, serum, plasma (heparin) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F21-G467

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human MMP8

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	261	1533	5210	248	1632	5016
Standard deviation	13.83	101.18	369.91	17.11	112.61	391.25
CV (%)	5.3%	6.6%	7.1%	6.9%	6.9%	7.8%

Background

Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	261	245	279	274	265	12.44	4.7%
Sample 2	1533	1322	1368	1496	1430	78.64	5.5%
Sample 3	5210	5280	4869	4875	5059	161.87	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	4317
Gene Name	CST3
UniProt ID	P22894
Protein Name	Neutrophil collagenase

Applications	ELISA
Research Area	Cardiovascular,Caspases,Cell Biology,Cell Type Markers,Neural Stem Cells,Stem Cells,Tags & Cell Markers,Tumor Associated

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000255	FEK0464	96 wells/kit	$499		Add to Cart

Human MMP-8 PicoKine® Quick ELISA Kit

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