Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0817

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	78	156	312	625	1,250	2,500	5,000
O.D.	0.033	0.096	0.150	0.312	0.430	0.798	1.315	1.913

Description

Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human KLK5 in cell culture supeatants, serum, plasma (heparin, EDTA), human milk and saliva. Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V23-S293

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human KLK5

Sample Type

Cell culture supeatants, Serum, Plasma, Human milk, Saliva

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	139	814	2166	151	752	2373
Standard deviation	5.69	35.81	121.29	9.96	38.35	151.87
CV(%)	4.1%	4.4%	5.6%	6.6%	5.1%	6.4%

Background

Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum coeum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	139	133	145	153	142	7.38	5.2%
Sample 2	814	803	781	813	802	13.63	1.7%
Sample 3	2166	2633	2239	2202	2310	189.42	8.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	25818
Gene Name	KLK5
UniProt ID	Q9Y337
Protein Name	Kallikrein-5

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000332	FEK0817	96 wells/kit	$499		Add to Cart

Human Kallikrein 5/KLK5 PicoKine® Quick ELISA Kit

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Reproducibility

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