JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit / QuickTest ELISA

Human HSP90B1 (Endoplasmin) QuickTest ELISA Kit

SKU : BHE10811330

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Wuhan Fine Biotech Co., Ltd

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	QT-EH1060
Description	Endoplasmin\|94 kDa glucose-regulated protein\|GRP-94\|Endoplasmic reticulum resident protein 99\|ERp99\|Heat shock protein 90 kDa beta member 1\|Tumor rejection antigen gp96\|HSP90B1\|GRP94\|TRA1\|Tra-1
Reactivity	Human
Detection Method	Double Antibody
Detection Range	78.125-5000pg/ml
Sample Type	Cell lysates, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants
Assay Type	Sandwich
Assay Duration	120 minutes
Kit Components	Coated ELISA plate, standard, ready to use capture/detection antibody, TMB, sample dilution buffer, wash buffer, stop solution
Recovery	80%~120%
Sensitivity	46.875pg/ml
Specificity	specifically binds with HSP90B1, no obvious cross reaction with other analogues.
Assay Procedure Summary	Step 1: Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C. Washing: Wash the plate twice without immersion. Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C. Washing: Wash the plate five times without immersion. Step 3: Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.) Step 4: Add 50ul stop solution. Read at 450nm immediately and calculate.
Stability	>95% at 4°C for 6 months

UniProt ID	P14625
Antigen	HSP90B1
Usage Notes	Microplate reader,incubator,automated plate washer or multi-channel pipette, precision single and multi-channel pipette,sterile tubes and eppendorf tubes, absorbent paper and loading slot, deionized or distilled water

Precision	Intra assay<8%,inter assay<10%

Storage	2-8 °C(for sealed)

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE10811330	QT-EH1060	48T	$315		Add to Cart
BHE10811330	QT-EH1060	96T	$450		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.