Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit

Mfr.No.

FEK0369

Sensitivity

<5 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.014	0.081	0.116	0.217	0.357	0.631	1.261	2.033

Description

Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human HGF in cell culture supeatants, serum and plasma (EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hepatocyte growth factor;Hepatopoietin-A;Scatter factor;SF;Hepatocyte growth factor alpha chain;Hepatocyte growth factor beta chain;HGF;HPTA;

Molecular Weight

83 kDa

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human HGF

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	169	1246	4183	168	1364	4239
Standard deviation	9.8	87.22	267.71	9.91	118.66	313.68
CV (%)	5.8%	7%	6.4%	5.9%	8.7%	7.4%

Background

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	169	159	165	152	161	6.41	3.9%
Sample 2	1246	1103	1132	1240	1180	63.61	5.3%
Sample 3	4183	3905	4308	3657	4013	252.15	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	3082
Gene Name	HGF
UniProt ID	P14210
Protein Name	Hepatocyte growth factor

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001902	FEK0369	96 wells/kit	$499		Add to Cart

Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit

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