Human EPCAM/Trop1 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0755

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.024	0.288	0.433	0.647	1.088	1.501	2.024	2.689

Description

Human EPCAM/Trop1 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human EPCAM in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Epithelial cell adhesion molecule;Ep-CAM;Adenocarcinoma-associated antigen;Cell surface glycoprotein Trop-1;Epithelial cell surface antigen;Epithelial glycoprotein;EGP;Epithelial glycoprotein 314;EGP314;hEGP314;KS 1/4 antigen;KSA;Major gastrointestinal tumor-associated protein GA733-2;Tumor-associated calcium signal transducer 1;CD326;EPCAM;GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1;

Molecular Weight

34932 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24 - K265

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human EPCAM

Subcellular Localization

Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	38	143	444	35	150	444
Standard deviation	1.63	6	33.74	1.82	7.5	37.29
CV (%)	4.3%	4.2%	7.6%	5.2%	5%	8.4%

Protein Function

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. .

Background

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	38	35	32	35	35	2.12	6%
Sample 2	143	161	140	157	150	8.92	5.9%
Sample 3	444	418	452	413	431	16.58	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4072
Gene Name	EPCAM
UniProt ID	P16422
Protein Name	Epithelial cell adhesion molecule
Tissue Specificity	Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Sequence	Belongs to the EPCAM family. Contains 1 thyroglobulin type-1 domain.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000170	FEK0755	96 wells/kit	$499		Add to Cart

Human EPCAM/Trop1 PicoKine® Quick ELISA Kit

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