Human CXCL13/BLC/BCA1 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0739

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.052	0.140	0.187	0.311	0.516	0.916	1.448	2.196

Description

Human CXCL13/BLC/BCA1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CXCL13/BLC/BCA1 in cell culture supeatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V23-R94

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human CXCL13

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	36	186	437	34	191	423
Standard deviation	2.19	9.67	28.84	2.1	12.6	33.41
CV(%)	6.1%	5.2%	6.6%	6.2%	6.6%	7.9%

Background

C-X-C motif chemokine 13(CXCL13), also known as B lymphocyte chemoattractant(BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	36	41	38	34	37	2.57	6.9%
Sample 2	186	197	198	189	193	5.2	2.7%
Sample 3	437	495	459	516	477	11.44	2.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	10563
Gene Name	CXCL13
UniProt ID	O43927

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000292	FEK0739	96 wells/kit	$499		Add to Cart

Human CXCL13/BLC/BCA1 PicoKine® Quick ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reproducibility

Reviews of Human CXCL13/BLC/BCA1 PicoKine® Quick ELISA Kit

Rate it now..!!