Human Clusterin PicoKine® Quick ELISA Kit

Mfr.No.

FEK0914

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.004	0.107	0.207	0.371	0.729	1.374	2.134	2.350

Description

Human Clusterin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CLU in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Clusterin;Aging-associated gene 4 protein;Apolipoprotein J;Apo-J;Complement cytolysis inhibitor;CLI;Complement-associated protein SP-40,40;Ku70-binding protein 1;NA1/NA2;Testosterone-repressed prostate message 2;TRPM-2;Clusterin beta chain;ApoJalpha;Complement cytolysis inhibitor a chain;Clusterin alpha chain;ApoJbeta;Complement cytolysis inhibitor b chain;CLU;APOJ, CLI, KUB1;AAG4;

Molecular Weight

52495 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)

Kit Components

Description Quantity Volume
Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
HRP-Linked Antibody 1 6ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Clusterin

Subcellular Localization

Isoform 1: Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1982	3789	21921	2148	4124	21708
Standard deviation	120.9	219.76	920.68	135.32	259.81	1085.4
CV (%)	6.1%	5.8%	4.2%	6.3%%	6.3%	5%

Protein Function

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers intealization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. .

Background

Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.(1) In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1982	1990	1978	1909	1964	32.47	1.6%
Sample 2	3789	4380	3818	4021	4002	235.83	5.8%
Sample 3	21921	20791	21896	19977	21146	814.81	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	1191
Gene Name	CLU
UniProt ID	P10909
Protein Name	Clusterin
Tissue Specificity	Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. .
Sequence	Belongs to the clusterin family.

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000167	FEK0914	96 wells/kit	$499		Add to Cart

Human Clusterin PicoKine® Quick ELISA Kit

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