Human APOE PicoKine® Quick ELISA Kit

Mfr.No.

FEK1455

Sensitivity

<50 pg/ml

Detection Range

3.12 ng/ml - 200 ng/ml

Reactivity

Human

Sample Volume

Concentration(ng/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.003	0.143	0.256	0.448	0.731	1.041	1.413	1.650

Description

Human APOE PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APOE in cell culture supeatants, serum, plasma (heparin, EDTA), saliva, milk and urine. Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: K19-H317

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human APOE

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Milk, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	7336	30862	101167	7284	33941	97075
Standard deviation	454.83	2129.47	7587.52	590.00	2884.98	8542.60
CV(%)	6.2%	6.9%	7.5%	8.1%	8.5%	8.8%

Background

Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-absorbance lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low absorbance lipoprotein receptor gene family. This protein is involved in Alzheimers disease and cardiovascular disease.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	7336	7271	6566	6791	6991	265.65	3.8%
Sample 2	30862	28096	32231	27122	29577	1449.27	4.9%
Sample 3	101167	109927	102578	89024	100674	6241.78	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	348
Gene Name	APOE
UniProt ID	P02649

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000272	FEK1455	96 wells/kit	$499		Add to Cart

Human APOE PicoKine® Quick ELISA Kit

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Reproducibility

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