High-Quality ELISA Kits for Accurate and Reliable Results

120 min Rapid Assay (Double antibody-Sandwich ELISA)​

-------Precision Detection Through Antibody-Affinity Tag Complex and HRP-Streptavidin Enzyme Reaction

​In the QuickTest ELISA, the capture antibody is conjugated to an affinity tag that binds to a specific antibody pre-coated on the plate. The Cap/Det Ab working solution is first added to each well, followed by the standards and samples. After mixing and incubation, if the target molecule is present, a complex forms that includes the capture antibody, the target, and a biotin-labeled detection antibody.

Following this step, unbound components are washed away, and HRP-Streptavidin is added to each well to bind with the biotin-labeled detection antibody. After incubation, another washing step is performed to remove any excess HRP-Streptavidin. TMB substrate is then added to the wells, which is catalyzed by the HRP enzyme to produce a blue color. Upon adding the stop solution, the blue color turns yellow. The optical density (OD) at 450 nm is measured using a microplate reader, and the target concentration is calculated by plotting a standard curve. The target concentration is directly proportional to the OD450 value.
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Assay Procedure Summary

Step 1: Take out the required strips, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37°C. Washing: Wash the plate twice without immersion.

Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37°C. Washing: Wash the plate five times without immersion.

Step 3: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C(Accurate TMB visualization control is required.).

​Step 4: Add 50ul stop solution. Read at 450nm immediately and calculate.

Minimized Errors​

Color coding has been added to distinguish the steps, and the reagents are pre-formulated for immediate use, eliminating the need for preparation, reducing operation time, and minimizing experimental errors.
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First Box (Blue): "Add standards and samples Color: Blue"
Second Box (Orange): "Add capture and detection antibodies Color: Orange"
Third Box (Blue): "Add TMB Color: Blue"
Fourth Box (Yellow): "Add stop solution Color: Yellow"