Recombinant Vesicular Stomatitis Virus (rVSV) pseudotyped with Ebola virus glycoprotein (rVSV-EBOV-GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV-pseudotyped virus containing the envelope glycoprotein of Ebola virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.
Expression
HEK-293T cells
Formulation
Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, Lglutamine and Penicillin/Streptomycin.
Pathogen Strain
Zaire
Target Pathogen
Ebola Virus
Virus Type
Filoviruses
Storage
Store at -80°C
Shipping
1 week
Notes
Recommended Dilution: 1:40 Luciferase Units for Assays: 30,000 Reporter: Luciferase
Reviews of rVSV-pseudotyped Ebola virus GP (rVSV-EBOV-GP)