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Home / Proteins & Peptide

ZAP70, GST-tag

SKU : BHP18200259

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BPS Bioscience

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Mfr.No.	40490
Description	Human ZAP70, alsoknown as ZAP-70, zeta-chain associatedprotein kinase, syk-related tyrosinekinase, SRK, or STD, GenBankAccession No. NM001079, full lengthwith N-terminal GST tag, MW = 96 kDa,expressed in a Baculovirus infected Sf9cell expression system. Host cell line: Sf9 cells Specific activity: 20 pmole/min/ug
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCL, pH 8.0,110 mM NaCl, 2.2 mM KCl, 3 mM DTT,20% glycerol and 1.6 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done inKinase buffer containing 0.2 mM DTTusing Poly-(Glu4:Tyr) substrate (0.2mg/ml) and 20 uM ATP. Reaction wasdone at 30°C for 45 min. Amount of ATPtransferred was calculated using Kinase-Glo reagent (Promega). Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank	NM_001079
Mw(kda)	96 kDa

Synonyms	ZAP-70, Zeta-Chain Associated Protein Kinase, Syk-Related Tyrosine Kinase, SRK, STD
Uniprot	P43403

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Ishaq, M., et al. J Immunol. 2007 Sep 15;179(6):3851-8. 2. Siggs, O., et al. Immunity. 2007 Dec;27(6):912-26.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200259	40490	10 ug	$390.50		Add to Cart

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