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Home / Proteins & Peptide

XIAP, FLAG-tag

SKU : BHP18200829

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BPS Bioscience

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Mfr.No.	80401
Description	Human X-linked inhibitor of apoptosis (XIAP), also known as IAP-like protein, API3, X-linked IAP, and BIRC4 (GenBank Accession No. NM_001167), a.a. 2-end, with N-terminal FLAG-tag, MW= 57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Immunogen Region	2-end
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful as an Ubiquitin ligase in conjunction with E1 and E2 for the study of enzyme kinetics. Ideal for Bir Domain binding and inhibition studies of Caspases 3 7, and 9. Also useful for screening inhibitors, and selectivity profiling.
Notes	Ligase Assay Conditions: 50 ul of reaction mix (4 mM ATP, 5mM MgCl₂, 1 mM DTT, E1 (0.5 ug), E2(2.8 ug), Biotin-Ubiquitin (0.5 ug) andXIAP (0-1.25 ug) in 50 mM Tris pH 7.4)is incubated for 30 min at 37°C.Transfer the reaction mixture to astreptavidin-coated plate and incubatefor 30 min at 37°C. Add antibody againstXIAP, incubate 1 hr. Then, addsecondary HRP-labeled antibody andincubate 30 min. Finally, add HRPchemiluminescent substrates andread luminescence. Bir Domain Assay Conditions: 50 mM Tris-HCl, pH 7.4, 25 mM NaCl,0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutesat room temperature. Fluorescencepolarization is measured at 475 nm/528 nmof exc/em wavelength. Avoid freeze/thaw cycles.
GenBank	NM_001167
Mw(kda)	57 kDa

Synonyms	X-linked inhibitor of apoptosis, XIAP, IAP-like protein, API3, X-linked IAP, BIRC4
Uniprot	P98170

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gu L. et al., Cancer Cell 15 (5), 363-375 (2009). 2. Deveraux Q.L. et al., Nature 388 (6639), 300-304 (1997).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200829	80401	20 ug	$456.50		Add to Cart

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