Human WDR5 (WD Repeat Domain 5), also known as SWD3 or BIG-3, GenBank Accession No. NM_017588, a.a. 2-334(end), with N-terminal Avi-His-tag, MW=39 kDa, expressed in Sf9 insect cells via a baculovirus expression system. This protein is enzymatically biotinylated using Avi-Tag technology. Biotinylation is confirmed to be>=90%.
host species: insect
Host species: insect
Host cell line: Sf9
Immunogen Region
2-334 (end)
Tag
Avi-His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles.
GenBank
NM_017588
Mw(kda)
39 kDa
Synonyms
WD repeat-containing protein 5, BIG3
Uniprot
P61964
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1-0.5%).
Reference
1. Trievel, R.C., Shilatifard, A. (2009). Nature Struct. Mol. Biol. 16 (7): 678-80.2. Wang, Y.Y., et al. (2010). Proc. Natl. Acad. Sci. USA 107(2):815-20.3. Odho, Z., et al. (2010). J. Biol. Chem. 285(43):32967-76.