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Home / Proteins & Peptide

VPS34, FLAG-tag

SKU : BHP18200291

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BPS Bioscience

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Mfr.No.	40690
Description	Human VPS34 (vacuolar protein sorting 34), GenBank Accession No. NM_002647, a.a. 2-888(end) with N-terminal FLAG-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW=98.2 kDa. Host cell line: Sf9 cells Specific activity: 140 pmol/min/ug.
Immunogen Region	2-888
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction was conducted in abuffer (40 ul) containing 40 mM Tris (pH 7.4), 10 mM MgCl₂, 2 mM MnCl₂, 0.1 mg/ml BSA, 100 uM ATP, 100 uM PI:PS substrate (BPS catalog #40560) and 0.6-5 ng/ul Vps34 at 30°C for 20 minutes. Kinase reaction was measured using ADP-Glo reagents (Promega). Avoid freeze/thaw cycles.
GenBank	NM_002647
Mw(kda)	98.2 kDa

Synonyms	VPS34, vacuolar protein sorting 34
Uniprot	Q8NEB9 P28221

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yan, Y., et al. (2009). Biochem J. 417(3):747-55. 2. Backer, J.M. (2008). Biochem J. 410(1):1-17. 3. JuhA¡sz, G., et al. (2008). J Cell Biol. 181(4):655-66.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200291	40690	20 ug	$445.50		Add to Cart

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