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Home / Proteins & Peptide

VEGF164, Rat (P. pastoris-expressed)

SKU : BHP18500141

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iXCells Biotechnologies

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Mfr.No.	IX02917, IX02917-1, IX02917-10, IX02917-2
Description	Vascular Endothelial Growth Factor A164 (VEGF-A164), a member of the cysteine knot growth factor(1), is one of major isoforms of VEGF-As. VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties(2). During maturation, rat VEGF-A is alternatively spliced to generate rVEGF-A120, rVEGF-A164 and rVEGF-A188(3) which correspond to hVEGF-A121, hVEGF-A165 and hVEGF-A189 in human, respectively (the numbers designate the amino acid residues). The active form of rVEGF-A164 is either a homodimeric or heterodimeric polypeptides which bind to the transmembrane tyrosine kinases receptors FLT1, FLK1 or KDR(4) or to the non-tyrosine kinase neuropilin receptors NRP1/2(5).Recombinant rat Vascular Endothelial Growth Factor A164 (rrVEGF-A164) produced in P. pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rrVEGF-A164 has a molecular mass of 38 kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	38 kDa, observed by non-reducing SDS-PAGE.
Source	P. pastoris
Host	Rat
Accession Number	P16612-2
Endotoxin Level	1 EU/ug, determined by LAL method.
Bioactivity	ED504 ng/ml, measured by cell proliferation assay of HUVEC.

Synonyms	Vascular Endothelial Growth Factor 164 (VEGF-164), VEGF, Vascular Permeability Factor (VPF)
Sequence	MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYPDEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQIMRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPENHCEPCSERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRCDKPRR

Purity	95% as analyzed by reducing SDS-PAGE.
Storage	Lyophilized recombinant rat Vascular Endothelial Growth Factor A164(rrVEGF-A164) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrVEGF-A164 should be stable up to 1 week at 4°C or up to 2 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500141-1	IX02917-1	1mg	$1872		Add to Cart
BHP18500141-2	IX02917-10	10ug	$107		Add to Cart
BHP18500141-3	IX02917-2	2ug	$49		Add to Cart

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