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Home / Proteins & Peptide

UTX (KDM6A), N-terminal FLAG-tag

SKU : BHP18200343

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Mfr.No.	50119
Description	Human UTX, also known as KDM6A (GenBank Accession No. NM_021140) (amino-acids 1-1401) with N-terminal FLAG-tag, MW = 155 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.6 pmol/min/ug
Immunogen Region	1-1401
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	0.5 uM of 3MeK27 H3 21-44(Biotin) is incubated for 1 hr incubation at RT with varying concentrations of UTX in 1x UTX Assay Buffer, then 5 uL of anti rabbit acceptor beads (1:250 dilution) + 5 uL of primary ab 6 (1:125 dilution) is added and incubated at RT for 30 min followed by 10 uL of streptavidin donor beads (1:125) for 30 min and alpha counts are read. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_021140
Mw(kda)	155 kDa

Synonyms	UTX, KDM6A
Uniprot	O15550

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary.

Reference	1. Greenfield A, et al. (1998). Hum. Mol. Genet. 7 (4): 737-42. 2. Agger K, et al. (2007). Nature 449 (7163): 731-4.Application Reference(s): Identification of the KDM2/7 histone lysine demethylase subfamily inhibitor and its antiproliferative activity (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200343	50119	20 ug	$522.50		Add to Cart

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