Human USP8 (Ubiquitin-Specific Protease 8) or UBPY, GenBank Accession No. NM_005154, a.a. 2-1118(end) with an N-terminal FLAG-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Specific activity: 6.5 pmole/min/ug
Immunogen Region
2-1118(end)
Tag
N-terminal FLAG-tag
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction was performed in50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP8 in a 50 uL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
Avoid freeze/thaw cycles.
GenBank
NM_005154
Mw(kda)
128 kDa
Synonyms
USP 8, Ubiquitin-Specific Protease 8, UBPY, USP-8
Uniprot
P40818
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Naviglio, S., et al. (1998). EMBO J. 17(12):3241-50. 2. Niendorf, S., et al. (2007). Mol Cell Biol. 727(13):5029-39. 3. Mizuno, E., et al. (2005). Mol. Biol. Cell. 16(11): 5163-74.