Human ubiquitin specific peptidase 7 (USP7) or HAUSP,GenBank Accession No. NM_003470,a.a. 2 - 1102 (end) with N-terminal His-Flag tag, MW = 130 kDa, expressed ina baculovirus infected Sf9 cell expression system.
Specific activity: 46.0 pmole/min/ug
Immunogen Region
2-1102 (end)
Tag
N-terminal His-FLAG-tags
Formulation
47.5 mM Tris-HCl, pH 8.0, 131 mM NaCl, 2.6 mM KCl, 90 mM imidazole, 5% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT. 0.5 mM EDTA, and 500 nM Ub-AMC. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, andemission at 460 nm.
Avoid freeze/thaw cycles.
GenBank
NM_003470
Mw(kda)
130 kDa
Synonyms
Ubiquitin specific peptidase 7, USP 7, HAUSP, USP-7
Uniprot
Q93009
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Fernandez-Montalvan, A., et al., FEBS J. 2007 Aug;274(16):4256-70. 2. Li, M., et al., Mol Cell. 2004 Mar 26;13(6):879-86.