Human USP2 (Ubiquitin-Specific Protease 2) or UPB41, GenBank Accession No. NM_004205, a.a. 259-605(end) with an N-terminal His-tag, MW=40.9 kDa, expressed in an E. coli cell expression system.
Specific activity: 247 pmol/min/ug
Immunogen Region
259-605(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 2.2 mM KCl, 15 mM beta-mercaptoethanol, 250 mM imidazole, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP2 in a 50 uL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured atexcitation = 340 nm, emission = 460 nm.