Human USP2 (Ubiquitin-Specific Protease 2) or UPB41, GenBank Accession No. NM_004205, a.a. 2-605(end) with an N-terminal FLAG-tag, MW=68 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Specific activity: 8.9 pmole/min/ug
Immunogen Region
2-605(end)
Tag
N-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/ul Flag peptide, 20% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP2 in a 50 uL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal wasmeasured at excitation =340 nm, emission =460 nm.