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Home / Proteins & Peptide

USP14, FLAG-tag

SKU : BHP18200822

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BPS Bioscience

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Mfr.No.	80364
Description	Human USP14 (Ubiquitin-Specific Protease 14) or TGT (tRNA-guanine transglycosylase), GenBank Accession No. NM_005151, a.a. 2-494(end) with an N-terminal FLAG-tag, MW=57 kDa, expressed in a baculovirus-infected Sf9 cell expression system. Specific activity: 5.7 pmole/min/ug
Immunogen Region	2-494(end)
Tag	N-terminal FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP14 in a 50 uL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm. Avoid freeze/thaw cycles.
GenBank	NM_005151
Mw(kda)	57 kDa

Synonyms	USP 14, Ubiquitin-Specific Protease 14, TGT, tRNA-guanine transglycosylase, USP-14
Uniprot	P54578

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Chen, P.C., et al. (2009). J. Neurosci. 29(35):10909-19. 2. Chen, Y.C., et al. (2010). RNA 16(5):958-68. 3. Nagai, A., et al. (2009). Biochem. Biophys. Res. Commun. 379(4):995-1000.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200822	80364	50 ug	$467.50		Add to Cart

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