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Home / Proteins & Peptide

UEV1A (UBE2V1), FLAG-tag

SKU : BHP18200815

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BPS Bioscience

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Mfr.No.	80325
Description	UEV1A (ubiquitin-conjugating enzyme E2 variant 1), also known as UBE2V1 or CROC-1 (GenBank Accession No. NM_021988), a.a. 2-170(end), with N-terminal FLAG-tag, MW=20 kDa, expressed in E. coli.
Immunogen Region	2-170(end)
Tag	N-terminal FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml Flag peptide, 10% glycerol, and 3 mM DTT.
Species	Human
Application	Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	50 ul reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 (0.5 ug), UEV1A or other E2, Biotin-Ubiquitin (0.5 ug) and Smurf2 (2 ug) in 50 mM Tris, pH 7.4) for 30 min at 37°C. Add to Streptavidin-coated plates for 30 min at 37°C. Incubate with anti-Smurf2, then HRP-labeled secondary antibody, and chemiluminescent detection. Avoid freeze/thaw cycles.
GenBank	NM_021988
Mw(kda)	20 kDa

Synonyms	UEV1A, UBE2V1, CROC-1, ubiquitin-conjugating enzyme E2 variant 1
Uniprot	Q13404

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Thomson TM, et al. (1998). FEBS Lett. 423 (1): 49-52. 2. Hau DD, et al. (2006). Biochemistry 45 (32): 9866-77. 3. Ma L, et al. (1998). Oncogene 17 (10): 1321-6.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200815	80325	100 ug	$319		Add to Cart

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