UCHL1, also known as Ubiquitin Carboxy-terminal Hydrolase L1 or PGP9.5 (GenBank Accession No. NM_004181), a.a. 2-224(end), with N-terminal His-tag, MW=23.4 kDa, expressed in E. coli.
Immunogen Region
2-224(end)
Tag
N-terminal His-tag
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL1. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, and emission at 460 nm.