Human Ubiquitin, also known as UBB, UBC, UBA52, and RPS27A. GenBank Accession No. NM_021009, a.a. 2-76, with C-terminal His-tag, expressed in an Sf9 expression system.
Immunogen Region
2-76
Tag
C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, Variable imidazole, and 20% glycerol
Species
Human
Application
Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_021009
Mw(kda)
10 kDa
Uniprot
P0CG48
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Reference
1. Bodine, S., et al., Science. 2001; 294(5547): 1704-1708.2. Bence, N., et al., Science. 2001; 292(5521): 1552-1555.