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Home / Proteins & Peptide

UBE3A, His-FLAG-tags

SKU : BHP18200807

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BPS Bioscience

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Mfr.No.	80302
Description	Human UBE3A (var1) (GenBank Accession No. NM_130838), a.a. 2-end, with N-terminal His-FLAG-tag, MW=99.8 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Immunogen Region	2-end
Tag	N-terminal His-FLAG-tags
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 80 ug/ml FLAGpeptide, 20% Glycerol and 3 mM DTT.
Species	Human
Application	Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	50 ul of reaction mixture 4 mM ATP, 5 mMMgCl₂, 1 mM DTT, E1 (0.5 ug), E2 (2.8 ug),Biotinylated Ubiquitin (1 ug) in 50 mMTris pH 7.4 was incubated for 30 min at37°C. Transfer the reaction mixture to a streptavidin-coated plate and incubate for 30 min at 37°C. Add antibody against UBE3A, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. Avoid freeze/thaw cycles.
GenBank	NM_130838
Mw(kda)	99.8 kDa

Synonyms	UBE3A, E6AP
Uniprot	Q05086

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Dhe-Paganon S., et al. Biomol NMR. 51(1-2):185-90. (2011). 2. Jung EM, etal. Int J Mol Med. 28(5):697-704. (2011).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200807	80302	20 ug	$407		Add to Cart

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